SHORT REPORT
Antimicrobial efficacy of herbal, homeopathic and conventional dentifrices against oral microflora: An in vitro study
Anusha Divvi 1  
,  
Pradeep R. Kumar 2  
,  
Prakasam Gopinath 3  
,  
 
 
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1
Department of Public Heath Dentistry, Indira Gandhi Institute of Dental Sciences, Sri Balaji Vidyapeeth, Pondicherry, India
2
Department of Community Dentistry, Saveetha Dental College and Hospitals, Chennai, India
3
Department of Microbiology, Royal Dental College, Palakkad, Kerala, India
CORRESPONDING AUTHOR
Shivashankar Kengadaran   

Department of Public Heath Dentistry, Indira Gandhi Institute of Dental Sciences, Sri Balaji Vidyapeeth, Pondicherry, 607402, India
Publication date: 2020-05-27
Submission date: 2019-12-18
Final revision date: 2020-05-09
Acceptance date: 2020-05-17
 
Popul. Med. 2020;2(May):14
KEYWORDS
TOPICS
ABSTRACT
Introduction:
This study compares the efficacy of herbal, homeopathic, and conventional dentifrices, on oral microflora using antibiotic susceptibility tests.

Methods:
Three strains of microorganisms, Streptococcus Mutans, Escherichia Coli, and Candida Albicans, were taken and incubated in Mutans media, Mueller Hilton agar, and Sabouraud Dextrose agar, respectively. Different dilutions (1:5, 1:10 and 1:15) of several brands of commercial toothpaste with different compositions were made. Sterile disks were incorporated with an equal amount of prepared toothpaste formulations using a micropipette. These disks were then placed equidistant to each other, and the plates were incubated for 24 hours.

Results:
The zone of inhibition against S. Mutans is found to be higher in homeopathic dentifrice 24 mm, 19 mm, and 20 mm, followed by herbal dentifrice 19 mm, 17 mm, and 13 mm, and the least by conventional dentifrice 17 mm, 15 mm and no inhibition, at 1:5, 1:10 and 1:15 dilution, respectively. The zone of inhibition against E. Coli is found to be higher in herbal dentifrice 18 mm, 17 mm and 16 mm followed by conventional dentifrice 18 mm, 17 mm, and 14 mm, and no inhibition by homeopathic dentifrice at 1:5, 1:10 and 1:15 dilution, respectively. Zone of inhibition against C. Albicans is found to be higher in herbal dentifrice 14 mm, 12 mm and 9 mm followed by conventional dentifrice 14 mm, 9 mm, and no inhibition, and the least by homeopathic dentifrice 10 mm, 9 mm and 7 mm, at 1:5, 1:10 and 1:15 dilutions, respectively.

Conclusions:
Toothpaste formulations containing homeopathic and natural antimicrobial agents were more effective in controlling the oral microflora compared to toothpaste containing synthetic antimicrobial agents like triclosan.

CONFLICTS OF INTEREST
The authors have completed and submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest and none was reported.
FUNDING
There was no source of funding for this research.
PROVENANCE AND PEER REVIEW
Not commissioned; externally peer reviewed.
 
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