Antibiogram fingerprinting of Vibrio species recovered from commercial fish specimens collected from key retailers.
1
Animal and Pasture Science, University of Fort Hare, Alice, South Africa
2
Department of Biotechnology and Biological Sciences, University of Fort Hare, Alice, South Africa
Popul. Med. 2026;8(Supplement Supplement 1):
ABSTRACT
INTRODUCTION:
Antimicrobial resistance (AMR) is a growing global public health challenge that compromises the effective treatment and prevention of infectious diseases. The current investigation focused on the fingerprints of antibiotic resistance in Vibrio species isolated from commercial fish specimens collected from prominent merchants in the Eastern Cape region of South Africa
METHODS:
Fresh-frozen fish samples were collected from selected retail stores. Presumptive Vibrio isolates were recovered using standard microbiological techniques and confirmed by polymerase chain reaction (pcr). Confirmed Vibrio isolates were tested for susceptibility to multiple antibiotics. Genotypic analysis was performed to detect resistance determinants, including blaTEM, mcr-1 to mcr-4, and tet genes. Multiple antibiotic resistance indices (mari) were calculated to assess contamination risk.
RESULTS:
a total of 190 presumed isolates; 92 isolates were verified to be members of the Vibrio genus and subsequently examined for antibiotic susceptibility. In the study, multiple drug resistance fingerprints were observed among Vibrio isolates, including resistance to Erythromycin (79%), Nitrofurantoin (40.2%), Colistin (34.8%), Ampicillin (31.5%), Cefotaxime (17.4%), Oxytetracycline (17.4%), Azithromycin (15.2%), Ceftazidime (15.2%), Trimethoprim (5.4%), Ciprofloxacin (3.3%), Imipenem (4%), and Ceftazidime (1.4%). mari values ranged from 0.25 to 0.5, exceeding the 0.2 threshold and indicating high-risk contamination. Genotypic screening detected blaTEM in 12% of resistant isolates, predominantly in V. vulnificus (75 % ) and V. parahaemolyticus (12%). mcr genes were present in 30.8 % of resistant isolates and tet genes in 17.4 %
CONCLUSIONS:
The presence of multidrug-resistant Vibrio species in commercial fish highlights a significant threat to food safety and public health. These findings emphasise the need for enhanced surveillance and sustainable antibiotic management in aquaculture and healthcare. The study underscores the importance of equitable and inclusive public health interventions to address antimicrobial resistance across food systems.
Antimicrobial resistance (AMR) is a growing global public health challenge that compromises the effective treatment and prevention of infectious diseases. The current investigation focused on the fingerprints of antibiotic resistance in Vibrio species isolated from commercial fish specimens collected from prominent merchants in the Eastern Cape region of South Africa
METHODS:
Fresh-frozen fish samples were collected from selected retail stores. Presumptive Vibrio isolates were recovered using standard microbiological techniques and confirmed by polymerase chain reaction (pcr). Confirmed Vibrio isolates were tested for susceptibility to multiple antibiotics. Genotypic analysis was performed to detect resistance determinants, including blaTEM, mcr-1 to mcr-4, and tet genes. Multiple antibiotic resistance indices (mari) were calculated to assess contamination risk.
RESULTS:
a total of 190 presumed isolates; 92 isolates were verified to be members of the Vibrio genus and subsequently examined for antibiotic susceptibility. In the study, multiple drug resistance fingerprints were observed among Vibrio isolates, including resistance to Erythromycin (79%), Nitrofurantoin (40.2%), Colistin (34.8%), Ampicillin (31.5%), Cefotaxime (17.4%), Oxytetracycline (17.4%), Azithromycin (15.2%), Ceftazidime (15.2%), Trimethoprim (5.4%), Ciprofloxacin (3.3%), Imipenem (4%), and Ceftazidime (1.4%). mari values ranged from 0.25 to 0.5, exceeding the 0.2 threshold and indicating high-risk contamination. Genotypic screening detected blaTEM in 12% of resistant isolates, predominantly in V. vulnificus (75 % ) and V. parahaemolyticus (12%). mcr genes were present in 30.8 % of resistant isolates and tet genes in 17.4 %
CONCLUSIONS:
The presence of multidrug-resistant Vibrio species in commercial fish highlights a significant threat to food safety and public health. These findings emphasise the need for enhanced surveillance and sustainable antibiotic management in aquaculture and healthcare. The study underscores the importance of equitable and inclusive public health interventions to address antimicrobial resistance across food systems.
| eISSN: | 2654-1459 |
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