Exploring Alstonia boonei-Associated Endophytic Fungi extracts as a Source of Antiplasmodial agents.
1
Malaria Unit, Centre Pasteur of Cameroon, Yaoundé, Cameroon
2
Microbiology, University of Yaoundé1, Yaoundé, Cameroon
Popul. Med. 2026;8(Supplement Supplement 1):
ABSTRACT
BACKGROUND:
Despite all strategies implemented to eradicate malaria, it remains one of the main causes of death worldwide. This is particularly related to the expansion of multidrug-resistant strains of Plasmodium species of which Plasmodium falciparum is the most prevalent in African continent [1]. This study aims to assess the antiplasmodial activity of endophytic fungi extracts isolated from Alstonia boonei.
METHODS:
Forty-one endophytic fungal isolates from the stem bark, roots, and leaves of Alstonia boonei, identified morphologically, were extracted with ethyl acetate [2]. These extracts were tested for antiplasmodial activity against P. falciparum strains: chloroquine-sensitive (3D7) and multidrug-resistant (Dd2). The 3D7 strain was exposed at ring, trophozoite, and schizont stages to the most active extracts for 24 hours. Parasitemia levels were assessed microscopically, and parasite morphology was examined via Giemsa-stained smears [3]. Chemical profiles of potent extracts were analyzed using phytochemical screening and UHPLC-LIT-MS/MS [4].
RESULTS:
The endophytic fungi extracts showed IC50s (inhibitory concentration 50%) ranging from 3,87 to 48,91 µg/mL against P. falciparum 3D7 and from 4,92 to 25,18 µg/mL against P. falciparum Dd2. After 24 h of extracts exposure, most of the treated parasites, at all stages, turned to the pyknotic form and a consumption of cytoplasm for trophozoite and shizont stages. the parasitemia obtained showed that the extracts acted on all stages, in particular on the ring and trophozoite stages. Ten families of secondary metabolites have been identified. It appears that all eight extracts contain alkaloids. Untargeted LC-MS/MS metabolomic analysis revealed a diversity of metabolic profiles among the fungal extracts. Some extracts (E37 and 15) have a distinct metabolome, while others (E4, 10 and 51) share significant similarities, suggesting a similar chemical composition.
CONCLUSIONS:
Endophytic fungi found in A. boonei exhibit antiplasmodial activities and deserve to be exploited further as sources of potential antimalarial agents.
Despite all strategies implemented to eradicate malaria, it remains one of the main causes of death worldwide. This is particularly related to the expansion of multidrug-resistant strains of Plasmodium species of which Plasmodium falciparum is the most prevalent in African continent [1]. This study aims to assess the antiplasmodial activity of endophytic fungi extracts isolated from Alstonia boonei.
METHODS:
Forty-one endophytic fungal isolates from the stem bark, roots, and leaves of Alstonia boonei, identified morphologically, were extracted with ethyl acetate [2]. These extracts were tested for antiplasmodial activity against P. falciparum strains: chloroquine-sensitive (3D7) and multidrug-resistant (Dd2). The 3D7 strain was exposed at ring, trophozoite, and schizont stages to the most active extracts for 24 hours. Parasitemia levels were assessed microscopically, and parasite morphology was examined via Giemsa-stained smears [3]. Chemical profiles of potent extracts were analyzed using phytochemical screening and UHPLC-LIT-MS/MS [4].
RESULTS:
The endophytic fungi extracts showed IC50s (inhibitory concentration 50%) ranging from 3,87 to 48,91 µg/mL against P. falciparum 3D7 and from 4,92 to 25,18 µg/mL against P. falciparum Dd2. After 24 h of extracts exposure, most of the treated parasites, at all stages, turned to the pyknotic form and a consumption of cytoplasm for trophozoite and shizont stages. the parasitemia obtained showed that the extracts acted on all stages, in particular on the ring and trophozoite stages. Ten families of secondary metabolites have been identified. It appears that all eight extracts contain alkaloids. Untargeted LC-MS/MS metabolomic analysis revealed a diversity of metabolic profiles among the fungal extracts. Some extracts (E37 and 15) have a distinct metabolome, while others (E4, 10 and 51) share significant similarities, suggesting a similar chemical composition.
CONCLUSIONS:
Endophytic fungi found in A. boonei exhibit antiplasmodial activities and deserve to be exploited further as sources of potential antimalarial agents.
| eISSN: | 2654-1459 |
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
