The importance of evaluating nonylphenol ethoxylate (Igepal Co-630) in the recovery of Toxoplasma gondii oocysts in sandy soil samples for environmental surveillance
1
Department of Pathology, Federal University of Espírito Santo, Vitória, Brazil
2
Food Center, Morphology and Microscopy Unit, Adolfo Lutz Institute, São Paulo, Brazil
Popul. Med. 2026;8(Supplement Supplement 1):
ABSTRACT
BACKGROUND:
soil is an important environmental reservoir of toxoplasma gondii oocysts and plays a relevant role in the epidemiology of toxoplasmosis. the characterization and standardization of methodologies for oocyst recovery from environmental matrices are essential to support environmental surveillance studies, establish associations with potential outbreaks, and inform public health actions.
OBJECTIVES:
to qualitatively evaluate the sensitivity of the igepal co-630 (0.1%) solution for the recovery of toxoplasma oocysts from artificially inoculated sandy soil samples using microscopy, aiming at its application in environmental studies relevant to public health surveillance.
METHODS:
toxoplasma oocysts were stained with dapi to improve visualization under fluorescence microscopy. different oocyst concentrations (1000, 500, 250, 100, 50, and 10 oocysts/ml) were prepared and inoculated into 5 g of sandy soil in 15 ml falcon tubes, in triplicate. samples were left in contact for 1 hour at room temperature. subsequently, 10 ml of igepal co 630 solution were added, followed by homogenization using a vortex for 2 minutes and centrifugation at 2000 xg for 20 minutes. the supernatant was discarded, 10 ml of sucrose solution was added, and the samples were centrifuged at 400 xg for 5 minutes. sucrose solution was added until a positive meniscus was formed, and a coverslip was placed on the tube. after 40 minutes, the coverslip was removed for microscopic examination. observations were performed using a primostar zeiss fluorescence microscope.
RESULTS:
the presence of toxoplasma gondii oocysts was observed in all tested concentrations, indicating that the igepal co 630 solution was able to qualitatively recover oocysts from sandy soil samples.
CONCLUSIONS:
the igepal co-630 (0.1%) solution represents a viable alternative for the recovery of toxoplasma gondii oocysts from sandy soil samples using microscopy. however, this method presents low sensitivity and should be evaluated in conjunction with molecular techniques to improve detection reliability.
soil is an important environmental reservoir of toxoplasma gondii oocysts and plays a relevant role in the epidemiology of toxoplasmosis. the characterization and standardization of methodologies for oocyst recovery from environmental matrices are essential to support environmental surveillance studies, establish associations with potential outbreaks, and inform public health actions.
OBJECTIVES:
to qualitatively evaluate the sensitivity of the igepal co-630 (0.1%) solution for the recovery of toxoplasma oocysts from artificially inoculated sandy soil samples using microscopy, aiming at its application in environmental studies relevant to public health surveillance.
METHODS:
toxoplasma oocysts were stained with dapi to improve visualization under fluorescence microscopy. different oocyst concentrations (1000, 500, 250, 100, 50, and 10 oocysts/ml) were prepared and inoculated into 5 g of sandy soil in 15 ml falcon tubes, in triplicate. samples were left in contact for 1 hour at room temperature. subsequently, 10 ml of igepal co 630 solution were added, followed by homogenization using a vortex for 2 minutes and centrifugation at 2000 xg for 20 minutes. the supernatant was discarded, 10 ml of sucrose solution was added, and the samples were centrifuged at 400 xg for 5 minutes. sucrose solution was added until a positive meniscus was formed, and a coverslip was placed on the tube. after 40 minutes, the coverslip was removed for microscopic examination. observations were performed using a primostar zeiss fluorescence microscope.
RESULTS:
the presence of toxoplasma gondii oocysts was observed in all tested concentrations, indicating that the igepal co 630 solution was able to qualitatively recover oocysts from sandy soil samples.
CONCLUSIONS:
the igepal co-630 (0.1%) solution represents a viable alternative for the recovery of toxoplasma gondii oocysts from sandy soil samples using microscopy. however, this method presents low sensitivity and should be evaluated in conjunction with molecular techniques to improve detection reliability.
| eISSN: | 2654-1459 |
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