Background and Objective: Several authors showed how the acquisition of healthy habits during childhood is essential for maintaining them during adulthood. Most children’s eating and physical behaviors are acquired in two main contexts: school and family. The present study aimed to explore families’ determinants as potential predictors of healthy lifestyle habits (adherence to the Mediterranean Diet (MD), Leisure Screen Time (LST), Moderate to Vigorous Physical Activity (MVPA), and engagement in organized sports (SE)) among a sample of primary school children. Methods: This cross-sectional study was conducted among 106 children (mean age 7,92 ±1.40; 50% male, 50% female) enrolled in a primary school in Imola (Italy). Data were collected from October to December 2019 using an interactive tool to assess parental features and children’s lifestyle (zoom-8 questionnaire) and actigraph accelerometers, to analyze children’s activity levels and sedentary behavior over a seven-day period. Multiple linear regression models with backward stepwise selection were employed to identify the independent factors associated with the continuous outcome variables. Results: Adherence to md (expressed by Kidmed index) was positively associated with fathers’ educational level (β=1.0; 95%ci 0.10, 1.9), parental sport participation (β=1.0; 95%ci 0.17, 1.9) and parental health literacy nutritional knowledge score (β=0.45; 95%ci 0.10, 0.81). Moreover, higher mothers’ educational level was negatively related to LST. Among the parental characteristics, nutritional knowledge is positively related to children’s average daily minutes of se (β=2.8; 95%ci 0.15, 5.4). However, MVPA was not related to parents’ characteristics. Conclusions: The present study emphasizes the importance of parental determinants that affect the lifestyle of primary school children. In this context, we can underline how health promotion actions aimed at the adult target can also have an important impact on the younger populations.