Background and Objective: Neurogenic lower urinary tract dysfunction has a considerable impact on the quality of life and daily activities of individuals and their families. Intermittent Urethral Catheterization (UIC) is the most used therapy in these cases. Aimed to evaluate the effectiveness of the educational video "Intermittent Urethral Catheterization in children: How to do it?" in the performance of UIC by parents and caregivers of children with neurogenic bladder. Methods: Quasi-experimental study, from June to October 2021, at Pediatric Hospital in Fortaleza, Brazil. The sample consisted of 30 mothers who were starting or in continuous use of the UIC, divided into two groups: Intervention Group (IG) and Comparison Group (CG). After signing the informed consent, the sociodemographic and clinical form and the King's Health Questionnaire (KHQ) was applied to both groups, as well as the pre-test, which consists of 10 questions about the content of the educational video. CG received routine guidelines from the outpatient clinic and the IG, in addition to the service guidelines, received information through the educational video. Results: Sample composed mostly of women who are married or in a stable union (83%), did not work (86.6%), income of up to R$1100.00 (67%), lived in the countryside (80%), p value 0.017. After the intervention, post-test detected a significant difference between the values of the groups, p = 0.013, where the intervention group had higher scores of correct answers with an average of 9.67 of the 10 questions. Regarding the KHQ, in the Emotions domain, the IG participants declared level 3, meaning emotional state moderately affected by the childs problem, with a standard deviation of 0.93. Conclusions: Intervention with an educational video is effective and contributes to family guidance, in addition to facilitating the understanding, visualization and execution of the procedure by parents/caregivers of children with this disorder.